Membrane-damaging and cytotoxic effects on human fibroblasts of alpha- and beta-hemolysins from Aeromonas hydrophila

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Separation of two hemolysins from Aeromonas hydrophila by isoelectric focusing.

Two hemolytic proteins (hemolysin I and II) with isoelectric points of 5.5 and 4.3 were purified by isoelectric focusing from the culture supernatant fluid of Aeromonas hydrophila. The purified hemolysins were unstable after dialysis and upon heating at 37 C and were inactivated by proteolytic enzymes. The two hemolysins were lethal for mice in a dose of 10 mug (hemolysin I) and 3 mug (hemolysi...

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 1981

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.34.3.949-956.1981